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TBX Agar (Tryptone Bile X-glucuronide Agar)

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

TBX Agar

(Tryptone Bile X-glucuronide Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ....................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Bile salts........................................................................................ 1.5g

X-β-D-glucuronide.................................................................... 0.075g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sigma-Aldrich.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes.

Use: For the detection and enumeration of E. coli in foodstuffs, animal

food, and water without further confirmation. E. coli colonies are col

ored blue-green. The presence of the enzyme β-D-glucuronidase differ

entiates most E. coli sp. from other coliforms. E. coli absorbs the chro

mogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-glucuronide. The

enzyme β-glucuronidase splits the bond between the chromophore 5-

bromo-4-chloro-3-indolyl and the β-D-glucuronide. Growth of accom

panying Gram-positive flora is largely inhibited by the use of bile salts.