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Tergitol 7 Broth

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

Tergitol 7 Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose ........................................................................................ 10.0g

Yeast extract.................................................................................. 3.0g

Pancreatic digest of casein............................................................ 2.5g

Peptic digest of animal tissue........................................................ 2.5g

Tergitol 7....................................................................................... 0.1g

Bromthymol Blue ....................................................................25.0mg

TTC solution ..............................................................................3.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

TTC Solution:

Composition per 100.0mL:

Triphenyltetrazolium chloride....................................................... 1.0g

Preparation of TTC Solution: Add triphenyltetrazolium chloride

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 997.0mL. Mix thoroughly. Gently heat

while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 25°C. Aseptically add 3.0mL of sterile TTC solu

tion. Mix thoroughly.

Use: For the isolation and cultivation of coliforms, Salmonella, and

other enteric bacteria.