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Tergitol 7 HiVeg Agar Base with TTC

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Lactose ........................................................................................ 10.0g

Plant peptone No. 3....................................................................... 5.0g

Yeast extract.................................................................................. 3.0g

Sodium heptadecyl sulfate ............................................................ 0.1g

Bromthymol Blue ..................................................................... 0.025g

TTC solution ..............................................................................5.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium, without TTC solution, is available as a pre

mixed powder from HiMedia.

TTC Solution:

Composition per 100.0mL:

Triphenyltetrazolium chloride..................................................... 0.05g

Preparation of TTC Solution: Add triphenyltetrazolium chloride

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 995.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C. Aseptically add 5.0mL of sterile TTC solution. Mix thorough

ly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the detection and enumeration of coliforms. Lactose-fer

menting bacteria appear as yellow colonies. Lactose-nonfermenting

bacteria appear as blue colonies. For the selective enumeration and

identification of coliform organisms, as per Indian Standard published

by BIS.