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Tetramethyl Ammonium Chloride Agar

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Tetramethyl ammonium chloride.................................................. 1.0g

Thiamine·HCl ............................................................................... 0.5g

Standard mineral base...................................................................1.0L

pH 6.5 ± 0.2 at 25°C

Standard Mineral Base:

Composition per liter:

(NH4)2SO4 .................................................................................... 1.0g

Phosphate buffer (1M solution, pH 6.8) ..................................40.0mL

Huntner’s vitamin-free mineral base .......................................20.0mL

Huntner’s Vitamin-Free Mineral Base:

Composition per liter:

MgSO4·7H2O............................................................................ 14.45g

Nitrilotriacetic acid ..................................................................... 10.0g

CaCl2·2H2O .............................................................................. 3.335g

FeSO4·7H2O............................................................................. 99.0mg

Metals “44”.............................................................................. 50.0mg

(NH4)6Mo7O24·4H2O ............................................................... 9.25mg

Preparation of Huntner’s Vitamin Free Mineral Base: Add

nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH

to 6.5 with KOH. Add remaining components. Add distilled/deionized

water to 1.0L. Adjust pH to 7.0.

Metals “44”:

Composition per 100.0mL:

ZnSO4·7H2O ........................................................................ 1095.0mg

FeSO4·7H2O........................................................................... 500.0mg

Ethylenediaminetetraacetic acid ............................................ 250.0mg

MnSO4·H2O........................................................................... 154.0mg

CuSO4·5H2O............................................................................ 39.2mg

Co(NO3)2·6H2O ....................................................................... 24.8mg

Na 2B4O7·10H2O...................................................................... 17.7mg

Preparation of Metals “44”: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Paracoccus kocurii.