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Tetrathionate Broth (FDA M145)

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

Tetrathionate Broth

(FDA M145)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1030.0mL:

Tetrathionate broth base................................................................1.0L

Iodine-potassium iodide solution.............................................20.0mL

Brilliant Green solution ...........................................................10.0mL

pH 8.4 ± 0.2 at 25°C

Tetrathionate Broth Base:

Composition per liter:

Na2S2O3·5H2O ............................................................................ 30.0g

CaCO3 ......................................................................................... 10.0g

Polypeptone™ .............................................................................. 5.0g

Bile salts........................................................................................ 1.0g

Preparation of Tetrathionate Broth Base: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. A slight precipitate will remain. Do

not autoclave. Cool to 25°C. Store at 4°C.

Iodine-Potassium Iodide Solution:

Composition per 20.0mL:

Iodine, resublimed ........................................................................ 6.0g

KI .................................................................................................. 5.0g

Preparation of Iodine-Potassium Iodide Solution: Add KI to

5.0mL of sterile distilled/deionized water. Mix thoroughly. Add iodine.

Mix thoroughly. Bring volume to 20.0mL with sterile distilled/deion

ized water.

Brilliant Green Solution:

Composition per 100.0mL:

Brilliant Green .............................................................................. 0.1g

Preparation of Brilliant Green Solution: Add Brilliant Green to

sterile distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly.

Preparation of Medium: Combine 1.0L of tetrathionate broth

base, 20.0mL of iodine-potassium iodide solution, and 10.0mL of Bril

liant Green solution. Mix thoroughly. Aseptically distribute into tubes

in 10.0mL volumes. Do not heat medium after it has been mixed.

Use: For the selective isolation and cultivation of Salmonella species

from foods.