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Tetrathionate Broth (TT Broth)

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

Tetrathionate Broth

(TT Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ...................................................................................... 30.0g

CaCO3 ......................................................................................... 10.0g

Proteose peptone........................................................................... 5.0g

Bile salts........................................................................................ 1.0g

Iodine solution .........................................................................20.0mL

pH 8.4± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Iodine Solution:

Composition per 20.0mL:

Iodine ............................................................................................ 6.0g

KI .................................................................................................. 5.0g

Preparation of Iodine Solution: Add iodine and KI to distilled/

deionized water and bring volume to 20.0mL. Mix thoroughly.

Preparation of Medium: Add components, except iodine solution,

to distilled/deionized water and bring volume to 980.0mL. Mix thor

oughly. Gently heat and bring to boiling. Do not autoclave. Cool to

40°C. Add 20.0mL of iodine solution. Mix thoroughly. Distribute into

tubes in 10.0mL volumes. Use medium the same day it is prepared.

Use: For the selective isolation and enrichment of Salmonella typhi

and other salmonellae from infectious material.