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Tetrathionate Reductase Medium

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per tube:

Solution I .................................................................................10.0mL

Solution III.................................................................................0.2mL

Solution II ..................................................................................0.1mL

Solution IV.................................................................................0.1mL

Solution I:

Composition per liter:

Na2HPO4·12H2O........................................................................... 3.6g

KH2PO4......................................................................................... 1.0g

NH4Cl ........................................................................................... 0.5g

Peptone ....................................................................................... 0.25g

Yeast extract................................................................................ 0.25g

MgSO4·7H2O.............................................................................. 0.03g

Preparation of Solution I: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 25°C.

Solution II:

Composition per 100.0mL:

CaCl2·2H2O .................................................................................. 0.1g

Ferric ammonium citrate............................................................. 0.05g

Preparation of Solution II: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 25°C.

Solution III:

Composition per 100.0mL:

Sodium succinate........................................................................ 15.0g

Preparation of Solution III: Add sodium succinate to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Gently

heat until dissolved. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 25°C.

Solution IV:

Composition per 100.0mL:

Na2S4O6·2H2O ............................................................................ 10.0g

Preparation of Solution IV: Add Na2S4O6·2H2O to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Sterilize

by filtration. Store at 4°C.

Preparation of Medium: To each tube containing 10.0mL of sterile

solution I, aseptically add 0.1mL of sterile solution II, 0.2mL of sterile

solution III, and 0.1mL of sterile solution IV. Mix thoroughly. Use im

mediately.

Use: For the cultivation and differentiation of hydrogen-oxidizing

bacteria based on their production of tetrathionate reductase.

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Tetrathionate Reductase Medium