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Tetrathionate Reductase Test Medium

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1025.0mL:

K2S4O6 .......................................................................................... 5.0g

Peptone water................................................................................1.0L

Bromthymol Blue (0.2% solution)...........................................25.0mL

pH 7.4 ± 0.2 at 25°C

Peptone Water:

Composition per liter:

Peptone........................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

Preparation of Peptone Water: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine components. Mix thoroughly.

Adjust pH to 7.4. Filter sterilize. Dispense into tubes in 1.0mL volumes

or into wells of sterile microculture plates for replica inoculation.

Use: For the cultivation and identification of Serratia species based on

their ability to reduce tetrathionate. Bacteria that reduce tetrathionate

turn the medium yellow.