培养基配方

您现在的位置：网站首页 >> 培养基配方

Tetrazolium Thallium Glucose Agar

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 14.0g

Beef extract ................................................................................. 10.0g

Peptone........................................................................................ 10.0g

Glucose solution ....................................................................100.0mL

2,3,5-Triphenyltetrazolium·HCl solution.................................10.0mL

Thallous acetate solution .........................................................10.0mL

2,3,5-Triphenyltetrazolium·HCl Solution:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium·HCl.................................................... 0.1g

Preparation of 2,3,5-Triphenyltetrazolium·HCl Solution:

Add 2,3,5-triphenyltetrazolium·HCl to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Autoclave for 7 min at 15 psi

pressure–121°C.

Glucose Solution:

Composition per 100.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Thallous Acetate Solution:

Composition per 10.0mL:

Thallous acetate ............................................................................ 1.0g

Preparation of Thallous Acetate Solution: Add thallous acetate

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components—except glucose solu

tion, 2,3,5-triphenyltetrazolium·HCl solution, and thallous acetate solu

tion—to distilled/deionized water and bring volume to 880.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glucose

solution, 2,3,5-triphenyltetrazolium·HCl solution, and thallous acetate

solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation of Streptococcus species.