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Thauera aromatica Medium

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition 1015.0mL:

Solution A ..............................................................................500.0mL

Solution B ..............................................................................500.0mL

Trace elements solution SL-10 ................................................10.0mL

Vitamin solution.........................................................................5.0mL

pH 7.5 ± 0.2 at 25°C

Solution A:

Composition per 500.0mL:

K2HPO4....................................................................................... 5.92g

KH2PO4..................................................................................... 0.816g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.5.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Solution B:

Composition per 500.0mL:

KNO3 ............................................................................................ 2.0g

Sodium benzoate......................................................................... 0.72g

NH4Cl ....................................................................................... 0.267g

MgSO4·7H2O............................................................................ 0.197g

CaCl2·2H2O .............................................................................. 0.025g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.5.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O................................................................................... 1.5g

CoCl2·6H2O ........................................................................... 190.0mg

MnCl2·4H2O .......................................................................... 100.0mg

ZnCl2........................................................................................ 70.0mg

Na2MoO4·2H2O ....................................................................... 36.0mg

NiCl2·6H2O.............................................................................. 24.0mg

H3BO3 ........................................................................................ 6.0mg

CuCl2·2H2O ............................................................................... 2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized

water and bring volume to 1.0L. Add remaining components. Mix thor

oughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to room temperature.

Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ........................................................................ 10.0mg

p-Aminobenzoic acid................................................................. 5.0mg

Lipoic acid ................................................................................. 5.0mg

Nicotinic acid............................................................................. 5.0mg

Riboflavin .................................................................................. 5.0mg

Thiamine·HCl ............................................................................ 5.0mg

Calcium DL-pantothenate........................................................... 5.0mg

Biotin ......................................................................................... 2.0mg

Folic acid ................................................................................... 2.0mg

Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Aseptically combine 500.0mL of sterile

solution A, 500.0mL of sterile solution B, 10.0 mL of sterile trace ele

ments solution SL-10, and 5.0 mL of sterile vitamin solution. Mix thor

oughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Thauera aromatica.

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Thauera aromatica Medium