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Thauera mechernichi Medium (DSMZ Medium 918)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thauera mechernichi Medium

(DSMZ Medium 918)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4 ..................................................................................... 4.20g

Na-acetate ................................................................................... 2.93g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.3g

MgSO4·7H2O ................................................................................ 0.1g

Trace elements solution .............................................................2.0mL

pH 7.1 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

Na2-EDTA................................................................................... 50.0g

CaCl2·2H2O................................................................................... 5.5g

MnCl2·4H2O................................................................................ 5.06g

FeSO4·7H2O.................................................................................. 5.0g

ZnSO4·7H2O ................................................................................. 2.0g

CoCl2·6H2O ................................................................................ 1.61g

CuSO4·5H2O ............................................................................... 1.57g

(NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 6.0.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thauera mechernichensis.