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Thayer-Martin Agar, Modified (MTM II) (Modified Thayer-Martin Agar)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thayer-Martin Agar, Modified

(MTM II)

(Modified Thayer-Martin Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 12.0g

Hemoglobin ................................................................................ 10.0g

Pancreatic digest of casein............................................................ 7.5g

Selected meat peptone .................................................................. 7.5g

NaCl .............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

CNVT inhibitor........................................................................10.0mL

Supplement solution ................................................................10.0mL

pH 7.2 ± 0.2 at 25°C

CNVT Inhibitor:

Composition per 10.0mL:

Colistin sulfate ...........................................................................7.5mg

Trimethoprim lactate..................................................................5.0mg

Vancomycin ...............................................................................3.0mg

Nystatin.................................................................................. 12,500U

Preparation of CNVT Inhibitor: Add components to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Supplement Solution:

Composition per liter:

Glucose ..................................................................................... 100.0g

L-Cysteine·HCl............................................................................ 25.9g

L-Glutamine................................................................................. 10.0g

L-Cystine ....................................................................................... 1.1g

Adenine......................................................................................... 1.0g

Nicotinamide adenine dinucleotide ............................................ 0.25g

Vitamin B12 ................................................................................... 0.1g

Thiamine pyrophosphate .............................................................. 0.1g

Guanine·HCl ............................................................................... 0.03g

Fe(NO3)3·6H2O........................................................................... 0.02g

p-Aminobenzoic acid................................................................ 0.013g

Thiamine·HCl ............................................................................ 3.0mg

Source: The supplement solution IsoVitaleX® enrichment is available

from BD Diagnostic Systems. This enrichment may be replaced by

supplement VX from BD Diagnostic Systems.

Preparation of Supplement Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except CNVT inhibitor

and supplement solution, to distilled/deionized water and bring volume

to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 10.0mL of sterile CNVT inhibitor

and 10.0mL of sterile supplement solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of Neisseria species from specimens containing

mixed flora of bacteria and fungi.

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Thayer-Martin Agar, Modified (MTM II) (Modified Thayer-Martin Agar)