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Thayer-Martin HiVeg Medium Base with Hemoglobin and Vitox Supplement

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thayer-Martin HiVeg Medium Base

with Hemoglobin and Vitox Supplement

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant special peptone .................................................................. 23.0g

Agar ............................................................................................ 13.0g

NaCl.............................................................................................. 5.0g

Starch ............................................................................................ 1.0g

Hemoglobin solution .............................................................250.0mL

Vitox supplement.....................................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without hemoglobin or Vitox supplement, is

available as a premixed powder from HiMedia.

Hemoglobin Solution:

Composition per 250.0mL:

Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to dis

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Vitox Supplement:

Composition per 10.0mL:

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl ......................................................................... 0.518g

L-Glutamine .................................................................................. 0.2g

L-Cystine................................................................................... 0.022g

Adenine sulfate ........................................................................... 0.01g

Nicotinamide adenine dinucleotide ........................................... 5.0mg

Cocarboxylase............................................................................ 2.0mg

Guanine·HCl .............................................................................. 0.6mg

Fe(NO3)3·6H2O.......................................................................... 0.4mg

p-Aminobenzoic acid............................................................... 0.26mg

Vitamin B12 ................................................................................ 0.2mg

Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitox Supplement: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except hemoblobin and

Vitox supplement, to distilled/deionized water and bring volume to

740.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add

250.0mL of sterile hemoglobin solution and 10.0mL of sterile Vitox

supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation and cultivation of fastidious microorganisms,

especially Neisseria species. For the selective isolation of gonococci

from pathological specimens.

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Thayer-Martin HiVeg Medium Base with Hemoglobin and Vitox Supplement