培养基配方

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Thayer-Martin Medium

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Hemoglobin ................................................................................ 10.0g

GC medium base....................................................................980.0mL

CNVT inhibitor........................................................................10.0mL

Supplement B...........................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a prepared medium in tubes from

BD Diagnostic Systems.

GC Medium Base:

Composition per 980.0mL:

Proteose peptone No. 3............................................................... 15.0g

Agar ............................................................................................ 10.0g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

pH 7.2 ± 0.2 at 25°C

Preparation of GC Medium Base: Add components of GC medi

um base and the hemoglobin to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

CNVT Inhibitor:

Composition per 10.0mL:

Colistin sulfate........................................................................... 7.5mg

Trimethoprim lactate.................................................................. 5.0mg

Vancomycin ............................................................................... 3.0mg

Nystatin.................................................................................. 12,500U

Preparation of CNVT Inhibitor: Add components to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: To 980.0mL of cooled sterile GC medium

base, aseptically add 10.0mL of sterile CNVT inhibitor and 10.0mL of

sterile supplement B. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the isolation and cultivation of fastidious microorganisms,

especially Neisseria species.