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Thayer-Martin Medium, Modified (Modified Thayer-Martin Agar)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thayer-Martin Medium, Modified

(Modified Thayer-Martin Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

GC agar base..........................................................................720.0mL

Hemoglobin solution .............................................................250.0mL

GC supplement ........................................................................30.0mL

pH 7.3 ± 0.2 at 25°C

GC Agar Base:

Composition per 720.0mL:

Special peptone........................................................................... 15.0g

Agar ............................................................................................ 10.0g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium

base to distilled/deionized water and bring volume to 720.0mL. Mix

thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution:

Composition per 250.0mL:

Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to dis

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

GC Supplement:

Composition per 30.0mL:

Yeast autolysate........................................................................... 10.0g

Glucose ......................................................................................... 1.5g

NaHCO3 ...................................................................................... 0.15g

Colistin sulfate ...........................................................................7.5mg

Trimethoprim lactate..................................................................5.0mg

Vancomycin ...............................................................................3.0mg

Nystatin.................................................................................. 12,500U

Preparation of GC Supplement: Add components to distilled/de

ionized water and bring volume to 30.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: To 720.0mL of cooled sterile GC agar

base, aseptically add 250.0mL of sterile hemoglobin solution and

30.0mL of sterile GC supplement. Mix thoroughly. Pour into sterile Pe

tri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of fastidious microor

ganisms, especially Neisseria species.

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Thayer-Martin Medium, Modified (Modified Thayer-Martin Agar)