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Thayer-Martin Medium, Modified (Modified Thayer-Martin Agar)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thayer-Martin Medium, Modified

(Modified Thayer-Martin Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

GC agar base..........................................................................730.0mL

Hemoglobin solution..............................................................250.0mL

Vitox supplement .....................................................................10.0mL

VCNT antibiotic solution.........................................................10.0mL

pH 7.3 ± 0.2 at 25°C

GC Agar Base:

Composition per 730.0mL:

Special peptone ........................................................................... 15.0g

Agar ............................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium

base in to distilled/deionized water and bring volume to 730.0mL. Mix

thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution:

Composition per 250.0mL:

Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to dis

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Vitox Supplement:

Composition per 10.0mL:

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl......................................................................... 0.518g

L-Glutamine.................................................................................. 0.2g

L-Cystine .................................................................................. 0.022g

Adenine sulfate ........................................................................... 0.01g

Nicotinamide adenine dinucleotide ........................................... 5.0mg

Cocarboxylase............................................................................ 2.0mg

Guanine·HCl .............................................................................. 0.6mg

Fe(NO3)3·6H2O.......................................................................... 0.4mg

p-Aminobenzoic acid............................................................... 0.26mg

Vitamin B12 ................................................................................ 0.2mg

Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitox Supplement: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

VCNT Antibiotic Solution:

Composition per 10.0mL:

Colistin methane sulfonate ........................................................ 7.5mg

Trimethoprim lactate.................................................................. 5.0mg

Vancomycin ............................................................................... 3.0mg

Nystatin.................................................................................. 12,500U

Preparation of VCNT Antibiotic Solution: Add components to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: To 730.0mL of cooled, sterile GC agar

base, aseptically add 250.0mL of sterile hemoglobin solution, 10.0mL

of sterile Vitox supplement, and 10.0mL of VCNT antibiotic solution.

Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the selective isolation and cultivation of fastidious microor

ganisms, especially Neisseria species.

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Thayer-Martin Medium, Modified (Modified Thayer-Martin Agar)