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培养基配方

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Thermococcus profundus Medium

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NaCl............................................................................................ 25.0g

Sulfur .......................................................................................... 10.0g

Peptone ......................................................................................... 5.0g

Yeast extract.................................................................................. 1.0g

Resazurin ................................................................................... 1.0mg

Salt base solution ..........................................................................1.0L

Na2S·9H2O solution.................................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Sulfur: Sterilize powdered elemental sulfur by

steaming for 3 hr at 0 psi pressure–100°C on 3 successive days.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Before use, neutralize to pH 7.0 with sterile HCl.

Salt Base Solution:

Composition per liter:

(NH4)2SO4..................................................................................... 1.3g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O .............................................................................. 0.25g

CaCl2·2H2O................................................................................. 0.07g

FeCl3·6H2O ................................................................................. 0.02g

Na2B4O7·10H2O.........................................................................4.5mg

MnCl2·4H2O...............................................................................1.8mg

ZnSO4·7H2O ............................................................................0.22mg

CuCl2·2H2O .............................................................................0.05mg

Na2MoO4·2H2O .......................................................................0.03mg

VOSO4 .....................................................................................0.03mg

CoSO4·7H2O ............................................................................0.02mg

Preparation of Salt Base Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Sparge

with 100% N2.

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components, except Na2S·9H2O solution, to salt base so

lution and bring volume to 1.0L. Mix thoroughly. Adjust medium pH to

7.2 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Imme

diately prior to use, aseptically and anaerobically add 10.0mL of sterile

Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically dis

tribute into sterile tubes or bottles.

Use: For the cultivation of Thermococcus profundus.

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Thermococcus profundus Medium