培养基配方

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Thermococcus stetteri Medium

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 25.0g

Sulfur .......................................................................................... 10.0g

Yeast extract.................................................................................. 3.0g

(NH4)2SO4..................................................................................... 1.3g

Peptone.......................................................................................... 0.5g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O .............................................................................. 0.25g

CaCl2·2H2O................................................................................. 0.07g

FeCl2·2H2O ................................................................................. 0.02g

NaB4O·10H2O............................................................................4.5mg

MnCl2·4H2O...............................................................................1.8mg

Resazurin ...................................................................................1.0mg

ZnSO4·7H2O ............................................................................0.22mg

CuCl2·2H2O .............................................................................0.05mg

NaMoO4·2H2O.........................................................................0.03mg

VOSO4·2H2O...........................................................................0.03mg

CoSO4 ......................................................................................0.01mg

Na2S·9H2O solution .................................................................10.0mL

pH 6.5 ± 0.2 at 25°C

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Na2S·9H2O so

lution, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 6.5. Do not autoclave. Sterilize by steaming at

100°C for 30 min on 3 consecutive days. Before inoculation, add

10.0mL of sterile Na2S·9H2O solution. Mix thoroughly.

Use: For the cultivation of Thermococcus stetteri.