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Thermoplasma acidophilum Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2SO4 .................................................................................. 1.32g

Yeast extract solution.................................................................... 1.0g

KH2PO4..................................................................................... 0.372g

MgSO4·7H2O............................................................................ 0.247g

CaCl2·2H2O .............................................................................. 0.074g

Glucose solution ......................................................................20.0mL

Yeast extract solution...............................................................10.0mL

Trace elements solution ...........................................................10.0mL

pH 1.0–2.0 at 25°C

Glucose Solution:

Composition per 20.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C.

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution:

Composition per liter:

FeCl3·6H2O ................................................................................. 1.93g

Na2B4O7·10H2O.......................................................................... 0.45g

MnCl2·4H2O................................................................................ 0.18g

ZnSO4·7H2O ............................................................................22.0mg

CuCl2·2H2O ...............................................................................5.0mg

VOSO4·5H2O.............................................................................3.8mg

Na2MoO4·2H2O ........................................................................ 3.0mg

CoSO4·7H2O ..............................................................................2.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except glucose solu

tion and yeast extract solution, to distilled/deionized water and bring

volume to 970.0mL. Mix thoroughly. Adjust pH to 1.0–2.0 with 10N

H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically

add 20.0mL of sterile glucose solution and 10.0mL of sterile yeast ex

tract solution. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks.

Use: For the cultivation and maintenance of Thermoplasma acidophi

lum.

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Thermoplasma acidophilum Medium