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Thermoplasma Broth

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermoplasma Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Basal solution.........................................................................500.0mL

Solution C ..............................................................................100.0mL

pH 2.0 ± 0.2 at 25°C

Basal Solution:

Composition per 500.0mL:

KH2PO4......................................................................................... 3.0g

Yeast extract.................................................................................. 1.0g

MgSO4·7H2O................................................................................ 0.5g

CaCl2·2H2O ................................................................................ 0.25g

(NH4)2SO4 .................................................................................... 0.2g

Preparation of Basal Solution: Add components to distilled/de

ionized water and bring volume to 500.0mL. Mix thoroughly. Adjust

pH to 2.0 with 10N H2SO4.

Solution C:

Composition per 100.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Solution C: Add glucose to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add 500.0mL of basal solution to

400.0mL of distilled/deionized water. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 55°C. Aseptically add 100.0mL of sterile glu

cose solution. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of Thermoplasma acidophi

lum and other Thermoplasma species.