培养基配方

您现在的位置：网站首页 >> 培养基配方

Thermoplasma Agar

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermoplasma Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Basal solution.........................................................................450.0mL

Solution B ..............................................................................450.0mL

Solution C ..............................................................................100.0mL

pH 2.0 ± 0.2 at 25°C

Basal Solution:

Composition per 500.0mL:

KH2PO4......................................................................................... 3.0g

Yeast extract.................................................................................. 1.0g

MgSO4·7H2O ................................................................................ 0.5g

CaCl2·2H2O................................................................................. 0.25g

(NH4)2SO4..................................................................................... 0.2g

Preparation of Basal Solution: Add components to distilled/de

ionized water and bring volume to 500.0mL. Mix thoroughly. Adjust

pH to 2.0 with 10N H2SO4. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 55°C.

Solution B:

Composition per 450.0mL:

Noble agar................................................................................... 12.0g

Preparation of Solution B: Add agar to distilled/deionized water

and bring volume to 450.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

55°C.

Solution C:

Composition per 100.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Solution C: Add glucose to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine the cooled, sterile

basal medium with sterile solution B and sterile solution C. Mix thor

oughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Thermoplasma acidophi

lum and other Thermoplasma species.