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Thermoplasma volcanium Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4......................................................................................... 3.0g

MgSO4·7H2O................................................................................ 1.0g

CaCl2·2H2O ................................................................................ 0.25g

(NH4)2SO4 .................................................................................... 0.2g

Glucose solution ......................................................................10.0mL

Yeast extract solution...............................................................10.0mL

pH 6.5 ± 0.2 at 25°C

Glucose Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except glucose solu

tion and yeast extract solution, to distilled/deionized water and bring

volume to 980.0mL. Mix thoroughly. Adjust pH to 2.0 with 10N

H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically

add 10.0mL of sterile glucose solution and 10.0mL of sterile yeast ex

tract solution. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks. Final pH should be 2.0–3.0.

Use: For the aerobic cultivation and maintenance of Thermoplasma

volcanium.