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Thermoproteus Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................500.0mL

Solution B ..............................................................................450.0mL

Solution C ................................................................................50.0mL

pH 4.8–5.6 at 25°C

Solution A:

Composition per 500.0mL:

Glucose ....................................................................................... 10.0g

FeSO4·7H2O.............................................................................. 0.556g

MgSO4·7H2O ............................................................................ 0.492g

CaSO4·2H2O ............................................................................. 0.344g

(NH4)2SO4................................................................................. 0.264g

Yeast extract.................................................................................. 0.2g

KH2PO4..................................................................................... 0.014g

Resazurin ...................................................................................1.0mg

Trace elements .........................................................................10.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Immediately filter

sterilize.

Trace Elements:

Composition per liter:

Na2B4O7·10H2O.......................................................................... 0.45g

MnCl2·4H2O................................................................................ 0.18g

ZnSO4·7H2O ............................................................................. 0.022g

CuCl2·2H2O ...............................................................................5.0mg

Na2MoO4·2H2O .........................................................................3.6mg

VOSO4·5H2O.............................................................................3.6mg

CoSO4·7H2O ..............................................................................1.2mg

Preparation of Trace Elements: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to

3.0 with H2SO4 to retard precipitation.

Solution B:

Composition per 450.0mL:

Sulfur .......................................................................................... 10.0g

Preparation of Solution B: Add sulfur to 450.0mL of distilled/de

ionized water. Autoclave for 30 min at 0 psi pressure–100°C on 3 con

secutive days.

Solution C:

Composition per 50.0mL:

Na2S·9H2O.................................................................................. 0.85g

Preparation of Solution C: Add Na2S·9H2O to distilled/deionized

water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine solutions A, B, and

C under 97% N2 + 3% H2. Adjust pH to 4.8–5.6 with H2SO4. Asepti

cally and anaerobically distribute into sterile tubes or flasks under 97%

N2 + 3% H2.

Use: For the cultivation and maintenance of Thermoproteus tenax and

other Thermoproteus species.

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Thermoproteus Medium