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Thermoproteus neutrophilus Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur, powdered........................................................................... 8.0g

(NH4)2SO4 .................................................................................... 1.3g

NaHCO3...................................................................................... 0.85g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O.............................................................................. 0.25g

CaCl2·2H2O ................................................................................ 0.07g

FeCl3·6H2O................................................................................. 0.02g

Na2B4·10H2O............................................................................. 4.5mg

MnCl2·4H2O .............................................................................. 1.8mg

Resazurin ................................................................................... 0.4mg

ZnSO4·7H2O ............................................................................ 0.22mg

CuCl2·2H2O ............................................................................. 0.05mg

Na2MoO4·2H2O ....................................................................... 0.03mg

VOSO4·2H2O........................................................................... 0.03mg

CoSO4 ...................................................................................... 0.01mg

Dithionite solution .....................................................................1.0mL

pH 6.5 ± 0.2 at 25°C

Dithionite Solution:

Composition per 10.0mL:

Na2S2O4 ...................................................................................... 0.25g

Preparation of Dithionite Solution: Add Na2S2O4 to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except sulfur,

NaHCO3, resazurin, and dithionite solution, to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Add sulfur and resazu

rin. Adjust pH to 6.5 with NaOH. Gently heat and bring to boiling.

Continue boiling for 5 min. Cool to room temperature while sparging

with 80% H2 + 20% CO2. Add NaHCO3. Mix thoroughly. Continue to

sparge with 80% H2 + 20% CO2 until pH reaches 6.5. Distribute anaer

obically 10.0mL of medium into 30.0mL serum bottles. Sterilize medi

um by heating at 85°C for 1 hr on 3 consecutive days. Prior to

inoculation, add 10.0μL of sterile dithionite solution to each bottle.

Use: For the aerobic cultivation and maintenance of Thermoproteus

neutrophilus.