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Thermosipho africanus Medium

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO3........................................................................................ 4.0g

Sodium acetate.............................................................................. 4.0g

Sodium formate............................................................................. 2.0g

Yeast extract.................................................................................. 1.0g

L-Cysteine·HCl.............................................................................. 0.5g

KH2PO4......................................................................................... 0.5g

Na2S·9H2O.................................................................................... 0.5g

MgSO4·7H2O ............................................................................... 0.4g

NaCl .............................................................................................. 0.4g

NH4Cl ........................................................................................... 0.4g

CaCl2·2H2O................................................................................. 0.05g

NiCl2·6H2O ..............................................................................24.0mg

FeSO4·7H2O.............................................................................. 2.0mg

Resazurin ...................................................................................1.0mg

Sludge fluid..............................................................................50.0mL

Fatty acid mixture ....................................................................20.0mL

Trace elements solution SL-10 ..................................................1.0mL

pH 6.7 ± 0.2 at 25°C

Sludge Fluid:

Composition per 100.0mL:

Sludge ....................................................................................100.0mL

Yeast extract.................................................................................. 0.4g

Preparation of Sludge Fluid: To 100.0mL of sludge from an an

aerobic digester, add 0.4g of yeast extract. Sparge with 100% N2 for a

few minutes. Incubate at 37°C for 24 hr. Centrifuge the sludge at

13,000 × g for 15 min. Decant the clear supernatant solution. Sparge

with 100% N2 for a few minutes. Store in screw-capped bottles at room

temperature in the dark.

Fatty Acid Mixture:

Composition per 20.0mL:

α-Methylbutyric acid .................................................................... 0.5g

Isobutyric acid............................................................................... 0.5g

Isovaleric acid ............................................................................... 0.5g

Valeric acid ................................................................................... 0.5g

Preparation of Fatty Acid Mixture: Add components to distilled/

deionized water and bring volume to 20.0mL. Mix thoroughly. Adjust

pH to 7.5 with concentrated NaOH.

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O ................................................................................... 1.5g

CoCl2·6H2O ...........................................................................190.0mg

MnCl2·4H2O...........................................................................100.0mg

ZnCl2........................................................................................70.0mg

Na2MoO4·2H2O .......................................................................36.0mg

NiCl2·6H2O ..............................................................................24.0mg

H3BO3 ........................................................................................6.0mg

CuCl2·2H2O ...............................................................................2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized

water and bring volume to 1.0L. Add remaining components. Mix thor

oughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–

121°C.

Preparation of Medium: Prepare and dispense medium anaerobi

cally under 80% N2 + 20% CO2. Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80%

N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thermosipho africanus.

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Thermosipho africanus Medium