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Thermovibrio Medium (DSMZ Medium 961)

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermovibrio Medium

(DSMZ Medium 961)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Synthetic seawater .................................................................970.0mL

Potassium nitrate solution........................................................10.0mL

Yeast extract solution...............................................................10.0mL

Na2S·9H2O solution.................................................................10.0mL

pH 6.5 ± 0.2 at 25°C

Synthetic Seawater:

Composition per liter:

NaCl............................................................................................ 27.7g

MgSO4·7H2O................................................................................ 7.0g

MgCl2·6H2O ................................................................................. 5.5g

CaCl2·2H2O ................................................................................ 0.75g

KCl.............................................................................................. 0.65g

KH2PO4......................................................................................... 0.5g

(NH4)2SO4 .................................................................................. 0.25g

NaBr.............................................................................................. 0.1g

H3BO3 ......................................................................................... 0.03g

SrCl2·6H2O...............................................................................15.0mg

Resazurin ...................................................................................1.0mg

KI ............................................................................................ 0.05mg

Preparation of Synthetic Seawater: Add components to distilled

water and bring volume to 1.0L. Mix thoroughly.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool

to room temperature.

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool

to room temperature.

Potassium Nitrate Solution:

Composition per 10.0mL:

KNO3 .......................................................................................... 0.33g

Preparation of Potassium Nitrate Solution: Add KNO3 to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Preparation of Medium: Gently heat and bring 1.0L synthetic sea

water to boiling. Boil for 3 min. Cool to 25°C while sparging with 80%

H2 + 20% CO2. Adjust pH to 6.8–7.0. Distribute 20.0mL aliquots into

100mL serum bottles under 80% H2 + 20% CO2. Autoclave for 15 min

at 15 psi pressure–121°C. Aseptically and anaerobically add per

20.0mL of medium, 0.2mL sterile potassium nitrate solution, 0.2mL

sterile yeast extract solution, and 0.2mL sterile Na2S·9H2O solution.

Mix thoroughly. Adjusted pH should be 6.5.

Use: For the cultivation of Thermovibrio ruber.

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Thermovibrio Medium (DSMZ Medium 961)