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Thermus Agar

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermus Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 28.0g

Pancreatic digest of casein............................................................ 2.5g

Yeast extract.................................................................................. 2.5g

Na2HPO4·12H2O ........................................................................ 0.43g

MgCl2·6H2O ................................................................................. 0.2g

Nitrilotriacetic acid ....................................................................... 0.1g

KH2PO4 ...................................................................................54.0mg

CaSO4·2H2O............................................................................40.0mg

Micronutrient solution ...............................................................1.0mL

Fe-citrate solution ......................................................................0.5mL

pH 7.2 ± 0.2 at 25°C

Micronutrient Solution:

Composition per liter:

MnSO4·H2O................................................................................ 2.28g

H3BO3........................................................................................... 0.5g

ZnSO4·7H2O................................................................................. 0.5g

CoCl2·6H2O .............................................................................45.0mg

CuSO4·5H2O............................................................................25.0mg

Na2MoO4·2H2O....................................................................... 25.0mg

Concentrated H2SO4..................................................................0.5mL

Preparation of Micronutrient Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Fe-Citrate Solution:

Composition per liter:

Fe-citrate.................................................................................. 24.5mg

Preparation of Fe-Citrate Solution: Add Fe-citrate to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add nitrilotriacetic acid to 100.0mL of

distilled/deionized water. Mix thoroughly. Adjust pH to 6.5 with KOH.

Add remaining components and bring volume to 1.0L with distilled/de

ionized water. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Thermus species.