培养基配方

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Thiamine Salts Medium

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4......................................................................................... 1.0g

FeSO4·7H2O................................................................................ 0.05g

MgSO4·7H2O.............................................................................. 0.02g

CaCl2........................................................................................... 0.02g

MnCl2·4H2O .............................................................................. 1.0mg

Na2MoO4·2H2O ......................................................................... 1.0mg

Thiamine·HCl solution ............................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Thiamine·HCl Solution:

Composition per 10.0mL:

Thiamine·HCl ............................................................................... 3.0g

Preparation of Thiamine·HCl Solution: Add thiamine·HCl to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except thiamine·HCl

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Adjust pH to 7.0 with KOH. Autoclave for 20 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add the sterile

thiamine·HCl solution. Mix thoroughly. Distribute into sterile tubes or

flasks.

Use: For the cultivation of ATCC strain 25589.