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Thiazole Medium

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thiazole Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 110.0mL:

Solution A..............................................................................100.0mL

Solution B ..................................................................................5.0mL

Solution C ..................................................................................5.0mL

pH 7.0 ± 0.2 at 25°C

Solution A:

Composition per 100.0mL:

Benzothiazole .......................................................................... 30.0mg

NaOH (10% solution)..............................................................90.0mL

Preparation of Solution A: Combine components. Mix thorough

ly. Adjust pH to 7.0 with concentrated HCl. Autoclave for 15 min at 15

psi pressure–121°C.

Solution B:

Composition per 50.0mL:

KH2PO4......................................................................................... 0.3g

Preparation of Solution B: Add KH2PO4 to distilled/deionized

water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Solution C:

Composition per 50.0mL:

MgSO4·7H2O........................................................................... 50.0mg

FeCl3 ........................................................................................ 10.0mg

CaCl2·2H2O ............................................................................... 6.0mg

Preparation of Solution C: Add components to distilled/deionized

water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 100.0mL of sterile

solution A with 5.0mL of sterile solution B and 5.0mL of sterile solu

tion C. Mix thoroughly.

Use: For the cultivation of unidentified bacteria DSMZ 8993 and

DSMZ 8994.