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Thioalkalivibrio halophilus Medium (DSMZ Medium 1014)

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thioalkalivibrio halophilus Medium

(DSMZ Medium 1014)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ......................................................................................... 175.0g

K2HPO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.5g

Magnesium chloride solution...................................................10.0mL

Magnesium sulfate solution .....................................................10.0mL

Thiosulfate solution .................................................................10.0mL

Bicarbonate solution ................................................................10.0mL

Trace element solution SL-4......................................................1.0mL

pH 8.2 ± 0.2 at 25°C

Magnesium Sulfate Solution:

Composition per 10.0mL:

MgSO4·7H2O .............................................................................. 0.25g

Preparation of Magnesium Sulfate Solution: Add MgSO4·7H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Magnesium Chloride Solution:

Composition per 10.0mL:

MgCl2·6H2O.................................................................................. 0.2g

Preparation of Magnesium Chloride Solution: Add MgCl2·6H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Thiosulfate Solution:

Composition per 10.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Adjust to pH 10.0. Filter sterilize.

Bicarbonate Solution:

Composition per 10.0mL:

NaHCO3 ........................................................................................ 4.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Trace Elements Solution SL-4:

Composition per liter:

EDTA ............................................................................................ 0.5g

FeSO4·7H2O.................................................................................. 0.2g

Trace elements solution SL-6 ..................................................... 100.0

Preparation of Trace Elements Solution SL-4: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-6:

Composition per liter:

H3BO3 ........................................................................................... 0.3g

CoCl2·6H2O .................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.1g

MnCl2·4H2O ............................................................................... 0.03g

Na2MoO4·H2O ............................................................................ 0.03g

NiCl2·6H2O................................................................................. 0.02g

CuCl2··2H2O................................................................................ 0.01g

Preparation of Trace Elements Solution SL-6: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 3.4.

Preparation of Medium: Add components, except magnesium

chloride, magnesium sulfate, thiosulfate, and bicarbonate solutions, to

distilled/deionized water and bring volume to 960.0mL. Mix thorough

ly. Adjust pH to 8.0–8.5. Dispense into screw-capped Erlenmeyer

flasks (fill to 1/10 volume). Autoclave for 30 min at 6 psi pressure–

110°C. Cool to room temperature. Aseptically add magnesium chlo

ride, magnesium sulfate, thiosulfate, and bicarbonate solutions. Mix

thoroughly.

Use: For the cultivation of Thioalkalivibrio halophilus.

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Thioalkalivibrio halophilus Medium (DSMZ Medium 1014)