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Thiobacillus Agar

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar, noble.................................................................................. 20.0g

Na2S2O3·5H2O .............................................................................. 5.0g

KH2PO4......................................................................................... 3.0g

CaCl2............................................................................................. 0.1g

MgCl2·6H2O.................................................................................. 0.1g

(NH4)2·Cl ...................................................................................... 0.1g

pH 4.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar, to dis

tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Adjust pH to 4.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C. Add agar to distilled/deionized water and bring volume

to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti

cally combine 500.0mL of the sterile basal medium with 500.0mL of

the sterile agar solution. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation of aciduric Thiobacillus species such as

Thiobacillus concretivorus and Thiobacillus intermedius.