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Thiobacillus Agar

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Ionagar No. 2 .............................................................................. 12.0g

Na2S2O3·5H2O ............................................................................ 10.0g

K2HPO4......................................................................................... 4.0g

KH2PO4......................................................................................... 4.0g

(NH4)2·SO4 ................................................................................... 0.1g

MgSO4·7H2O................................................................................ 0.1g

CaCl2............................................................................................. 0.1g

FeCl3·6H2O................................................................................ 2.0mg

MnSO4·4H2O............................................................................. 2.0mg

pH 6.6 ± 0.2 at 25°C

Source: Ionagar No. 2 is available from Oxoid Unipath.

Preparation of Medium: Add components, except the agar, to dis

tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.

Adjust the pH to 6.6. Add the agar. Bring volume to 1.0L with distilled/

deionized water. Gently heat and bring to boiling. Distribute into tubes

or flasks. Autoclave for 20 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation of nonaciduric Thiobacillus species such as

Thiobacillus neapolitanus, Thiobacillus novellus, and Thiobacillus thi

oparus.