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Thiobacillus Agar I for Acidophilic Thiobacillus

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL

Solution B ..............................................................................500.0mL

pH 4.2 ± 0.2 at 25°C

Solution A:

Composition per 500.0mL:

Na2S2O3........................................................................................ 5.0g

KH2PO4 ........................................................................................ 3.0g

CaCl2 ............................................................................................ 0.1g

MgCl2·6H2O ................................................................................. 0.1g

NH4Cl ........................................................................................... 0.1g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Adjust pH to 4.2 with 1N HCl.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C.

Solution B:

Composition per 500.0mL:

Agar, noble.................................................................................. 20.0g

Preparation of Solution A: Add agar to distilled/deionized water

and bring volume to 500.0mL. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile

solution A and 500.0mL of sterile solution B. Combine the two solu

tions while still hot. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation of Thiobacillus thiooxidans.