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Thiobacillus albertis Agar

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 500.0mL:

Solution A..............................................................................250.0mL

Solution B ..............................................................................250.0mL

pH 4.0 ± 0.2 at 25°C

Solution A:

Composition per 250.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

KH2PO4......................................................................................... 3.0g

MgSO4·7H2O................................................................................ 0.5g

(NH4)2SO4 .................................................................................... 0.4g

CaCl2·2H2O ................................................................................ 0.25g

FeSO4·7H2O............................................................................ 10.0mg

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 250.0mL. Mix thoroughly. Adjust pH to 4.0.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 250.0mL:

Agar ............................................................................................ 15.0g

Preparation of Solution B: Add agar to distilled/deionized water

and bring volume to 250.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Preparation of Medium: Aseptically combine 250.0mL of sterile

solution A with 250.0mL of sterile solution B. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thiobacillus albertis.