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培养基配方

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Thiobacillus caldus Agar

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution E ..............................................................................500.0mL

Solution A..............................................................................460.0mL

Solution D................................................................................20.0mL

Solution B ................................................................................10.0mL

Solution C ................................................................................10.0mL

pH 2.5 ± 0.2 at 25°C

Solution A:

Composition per 460.0mL:

Na2SO4·10H2O ............................................................................. 3.2g

(NH4)2SO4 .................................................................................... 3.0g

MgSO4·7H2O................................................................................ 0.5g

KCl................................................................................................ 0.1g

K2HPO4.................................................................................... 50.0mg

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 460.0mL. Mix thoroughly. Adjust pH to

1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Cool

and maintain above 60°C.

Solution B:

Composition per 10.0mL:

FeCl3·6H2O.............................................................................. 11.0mg

Ca(NO3)2·4H2O....................................................................... 10.0mg

H3BO3........................................................................................ 2.0mg

MnSO4·H2O............................................................................... 2.0mg

ZnSO4·7H2O.............................................................................. 0.9mg

Na2MoO4·2H2O......................................................................... 0.8mg

CoCl2·6H2O............................................................................... 0.6mg

CuSO4·5H2O.............................................................................. 0.5mg

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution C:

Composition per 10.0mL:

Glucose ....................................................................................... 0.45g

Preparation of Solution C: Add glucose to distilled/deionized wa

ter and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution D:

Composition per 20.0mL:

Na2S4O6 ...................................................................................... 0.77g

Preparation of Solution D: Add Na2S4O6 to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Solution E:

Composition per 500.0mL:

Phytagel™ (Gellan gum; available from Sigma Chemical Co.) 15.0g

Preparation of Solution E: Add phytagel to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool and maintain above 60°C.

Preparation of Medium: Maintain solutions A and E above 60°C

to prevent rapid gelling of medium. Aseptically combine 460.0mL of

sterile solution A with 10.0mL of sterile solution B, 10.0mL of sterile

solution C, 20.0mL of sterile solution D, and 500.0mL of sterile solu

tion E. Mix throughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the heterotrophic cultivation and maintenance of Thiobacil

lus caldus.

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Thiobacillus caldus Agar