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培养基配方

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Thiobacillus caldus Broth

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................960.0mL

Solution D ................................................................................20.0mL

Solution B ................................................................................10.0mL

Solution C ................................................................................10.0mL

pH 2.5 ± 0.2 at 25°C

Solution A:

Composition per 960.0mL:

Na2SO4·10H2O.............................................................................. 3.2g

(NH4)2SO4..................................................................................... 3.0g

MgSO4·7H2O ................................................................................ 0.5g

KCl................................................................................................ 0.1g

K2HPO4....................................................................................50.0mg

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to

1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B:

Composition per 10.0mL:

FeCl3·6H2O.............................................................................. 11.0mg

Ca(NO3)2·4H2O.......................................................................10.0mg

H3BO3........................................................................................2.0mg

MnSO4·H2O...............................................................................2.0mg

ZnSO4·7H2O..............................................................................0.9mg

Na2MoO4·2H2O.........................................................................0.8mg

CoCl2·6H2O ...............................................................................0.6mg

CuSO4·5H2O..............................................................................0.5mg

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution C:

Composition per 10.0mL:

Glucose ....................................................................................... 0.45g

Preparation of Solution C: Add glucose to distilled/deionized wa

ter and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution D:

Composition per 20.0mL:

Na2S4O6 ...................................................................................... 0.77g

Preparation of Solution D: Add Na2S4O6 to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 960.0mL of sterile

solution A with 10.0mL of sterile solution B, 10.0mL of sterile solution

C, and 20.0mL of sterile solution D. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the heterotrophic cultivation and maintenance of Thiobacil

lus caldus.

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Thiobacillus caldus Broth