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Thiobacillus ferrooxidans Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Al2(SO4)3·12H2O .......................................................................... 1.4g

NaCl .............................................................................................. 1.0g

KH2PO4......................................................................................... 0.4g

MgSO4·7H2O ................................................................................ 0.1g

(NH4)2SO4..................................................................................... 0.1g

CaCl2........................................................................................... 0.03g

MnSO4·4H2O .............................................................................. 0.02g

FeSO4·7H2O solution.............................................................100.0mL

FeSO4·7H2O Solution:

Composition per 100.0mL:

FeSO4·7H2O................................................................................ 10.0g

H2SO4, concentrated ................................................................0.09mL

Preparation of FeSO4·7H2O Solution: Add FeSO4·7H2O and

H2SO4 to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except FeSO4·7H2O

solution, to distilled/deionized water and bring volume to 900.0mL.

Mix thoroughly. Gently heat and bring to boiling. Distribute into flasks

in 90.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 25°C. Aseptically add 10.0mL of sterile FeSO4·7H2O solution

to each flask. Mix thoroughly.

Use: For the cultivation of Thiobacillus ferrooxidans.