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Thiobacillus ferrooxidans Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution I................................................................................400.0mL

Solution III.............................................................................400.0mL

Solution II ..............................................................................200.0mL

Solution I:

Composition per 500.0mL:

K2HPO4......................................................................................... 0.5g

MgSO4·7H2O ................................................................................ 0.5g

(NH4)2SO4..................................................................................... 0.5g

H2SO4 (1N solution) ..................................................................5.0mL

Preparation of Solution I: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution II:

Composition per liter:

FeSO4·7H2O.............................................................................. 167.0g

1N H2SO4 .................................................................................50.0mL

Preparation of Solution II: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Warm

to 45°–50°C.

Solution III:

Composition per liter:

Agar ............................................................................................ 10.0g

Preparation of Solution III: Add agar to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Aseptically combine 400.0mL of sterile

solution I, 200.0mL of sterile solution II, and 400.0mL of sterile solu

tion III. Mix thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the isolation and cultivation of Thiobacillus ferrooxidans.