培养基配方

您现在的位置：网站首页 >> 培养基配方

Thiobacillus halophilus Broth

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................900.0mL

Solution B ..............................................................................100.0mL

pH 7.3 ± 0.2 at 25°C

Solution A:

Composition per 900.0mL:

NaCl ............................................................................................ 50.0g

Na2S2O3·5H2O .............................................................................. 5.0g

NH4Cl ........................................................................................... 0.4g

MgSO4·7H2O ................................................................................ 0.1g

Phenol Red (saturated aqueous solution).................................12.5mL

Trace metals solution ...............................................................10.0mL

Trace Metals Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

NaOH .......................................................................................... 11.0g

ZnSO4·7H2O............................................................................... 11.0g

CaCl2·2H2O ................................................................................ 7.34g

FeSO4·7H2O ................................................................................. 5.0g

MnCl2·4H2O ................................................................................. 2.5g

CoCl2·6H2O.................................................................................. 0.5g

(NH4)6Mo7O24·4H2O ................................................................... 0.5g

CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Metals Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Adjust pH to 6.0. Mix

thoroughly.

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Solution B:

Composition per 100.0mL:

Na2HPO4·2H2O............................................................................. 7.9g

KH2PO4......................................................................................... 1.5g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 900.0mL of sterile

solution A with 100.0mL of sterile solution B. Mix thoroughly. Asep

tically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus halophilus.