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培养基配方

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Thiobacillus halophilus Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution B ..............................................................................900.0mL

Solution A..............................................................................100.0mL

pH 7.3 ± 0.2 at 25°C

Solution B:

Composition per 900.0mL:

NaCl............................................................................................ 50.0g

Agar, noble.................................................................................. 15.0g

Na2S2O3·5H2O .............................................................................. 5.0g

NH4Cl ........................................................................................... 0.4g

Phenol Red............................................................................... 10.0mg

Hutner's basal salts solution.....................................................20.0mL

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C.

Hutner’s Basal Salts Solution:

Composition per liter:

MgSO4·7H2O.............................................................................. 29.7g

Nitrilotriacetic acid ..................................................................... 10.0g

CaCl2·2H2O .............................................................................. 3.335g

FeSO4·7H2O............................................................................. 99.0mg

(NH4)6MoO7O24·4H2O ............................................................ 9.25mg

“Metals 44”..............................................................................50.0mL

“Metals 44”:

Composition per 100.0mL:

ZnSO4·7H2O ............................................................................. 1.095g

FeSO4·7H2O.................................................................................. 0.5g

Sodium EDTA............................................................................. 0.25g

MnSO4·H2O ............................................................................. 0.154g

CuSO4·5H2O............................................................................ 39.2mg

Co(NO3)2·6H2O ....................................................................... 24.8mg

Na2B4O7·10H2O....................................................................... 17.7mg

Preparation of “Metals 44”: Add sodium EDTA to distilled/de

ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few

drops of concentrated H2SO4 to retard precipitation of heavy metal

ions. Add remaining components. Mix thoroughly. Bring volume to

100.0mL with distilled/deionized water.

Preparation of Hutner’s Basal Salts Solution: Add nitrilotria

cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5

with KOH. Add remaining components. Add distilled/deionized water

to 1.0L. Adjust pH to 6.8.

Solution A:

Composition per 100.0mL:

Na2HPO4 ....................................................................................... 6.3g

KH2PO4......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 900.0mL of sterile

solution B with 100.0mL of sterile solution A. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thiobacillus halophilus.

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Thiobacillus halophilus Medium