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Thiobacillus Medium (ATCC Medium 64)

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus Medium

(ATCC Medium 64)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 500.0mL:

Solution A ..............................................................................400.0mL

Solution B ..............................................................................100.0mL

pH 2.8 ± 0.2 at 25°C

Solution A:

Composition per 400.0mL:

(NH4)2SO4..................................................................................... 0.4g

KH2PO4......................................................................................... 0.2g

MgSO4·7H2O .............................................................................. 0.08g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 400.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution B:

Composition per 100.0mL:

FeSO4·7H2O................................................................................ 10.0g

H2SO4 (1N solution) ..................................................................1.0mL

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Aseptically add 100.0mL of cooled ster

ile solution B to 400.0mL of cooled sterile solution A. Mix thoroughly.

Adjust pH to 2.8. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of a variety of Thiobacillus

species.