培养基配方

您现在的位置：网站首页 >> 培养基配方

Thiobacillus Medium (ATCC Medium 125)

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus Medium

(ATCC Medium 125)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur .......................................................................................... 10.0g

KH2PO4......................................................................................... 3.0g

MgSO4·7H2O ................................................................................ 0.5g

CaCl2........................................................................................... 0.25g

(NH4)2SO4..................................................................................... 0.2g

FeSO4·7H2O...............................................................................5.0mg

Preparation of Medium: Add components, except sulfur, to tap

water and bring volume to 1.0L. Mix thoroughly. Add 1.0g of sulfur to

each of 10 flasks. Distribute the broth in 100.0mL volumes into the

flasks. Pour the broth down the side of the flask so that the sulfur is not

wetted. Autoclave for 30 min at 0 psi pressure–100°C on 3 consecutive

days. Be sure that sulfur remains on the surface of the broth during the

sterilization.

Use: For the cultivation and maintenance of a variety of Thiobacillus

species.