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Thiobacillus Medium (ATCC Medium 426)

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus Medium

(ATCC Medium 426)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3·5H2O ............................................................................ 10.0g

Na2HPO4·7H2O............................................................................. 7.9g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.3g

MgSO4·7H2O................................................................................ 0.1g

Phenol Red................................................................................. 2.0mg

Trace metals solution.................................................................5.0mL

pH 8.5 ± 0.2 at 25°C

Trace Metals Solution:

Composition per liter:

EDTA.......................................................................................... 50.0g

ZnSO4 ......................................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2.......................................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2 .......................................................................................... 1.61g

CuSO4 ......................................................................................... 1.57g

(NH4)2MoO4·4H2O....................................................................... 1.1g

Preparation of Trace Metals Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 6.0 with KOH.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.5 with

10% Na2CO3. Distribute into tubes or flasks. Autoclave for 15 min at

15 psi pressure–121°C. Adjust pH to 8.5 with sterile 10% Na2CO3 if

necessary. The broth should be pink.

Use: For the cultivation and maintenance of a variety of Thiobacillus

species.