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Thiobacillus Medium (ATCC Medium 528)

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus Medium

(ATCC Medium 528)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ...................................................................................... 10.0g

Yeast extract.................................................................................. 5.0g

NH4Cl ........................................................................................... 1.0g

K2HPO4......................................................................................... 0.6g

MgCl2............................................................................................ 0.5g

KH2PO4......................................................................................... 0.4g

MgSO4 .......................................................................................... 0.3g

Bromthymol Blue ....................................................................... 0.03g

FeCl3 ........................................................................................... 0.02g

Heavy metal solution ...............................................................30.0mL

pH 6.8 ± 0.2 at 25°C

Heavy Metal Solution:

Composition per liter:

Ethylenediamine tetraacetate ........................................................ 1.5g

FeSO4·7H2O.................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.1g

MnCl2·4H2O................................................................................ 0.02g

Modified Hoagland trace elements solution ..............................6.0mL

Preparation of Heavy Metal Solution: Add EDTA to approxi

mately 900.0mL of distilled/deionized water. Dissolve by adjusting pH

to 7.0 with NaOH. Bring volume to 1.0L with distilled/deionized wa

ter.

Modified Hoagland Trace Elements Solution:

Composition per 3.6L:

H3BO3 ......................................................................................... 11.0g

MnCl2·4H2O.................................................................................. 7.0g

AlCl3 ............................................................................................. 1.0g

CoCl2............................................................................................. 1.0g

CuCl2............................................................................................. 1.0g

KI .................................................................................................. 1.0g

NiCl2 ............................................................................................. 1.0g

ZnCl2............................................................................................. 1.0g

BaCl2............................................................................................. 0.5g

KBr................................................................................................ 0.5g

LiCl ............................................................................................... 0.5g

Na2MoO4....................................................................................... 0.5g

SeCl4 ............................................................................................. 0.5g

SnCl2·2H2O................................................................................... 0.5g

NaVO3·H2O .................................................................................. 0.1g

Preparation of Modified Hoagland Trace Elements Solu

tion: Prepare each component as a separate solution. Dissolve each

salt in approximately 100.0mL of distilled/deionized water. Adjust the

pH of each solution to below 7.0. Combine all the salt solutions and

bring the volume to 3.6L with distilled/deionized water. Adjust the pH

to 3–4. A yellow precipitate may form after mixing. After a few days,

it will turn into a fine white precipitate. Mix the solution thoroughly .

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of a variety of Thiobacillus

species.

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Thiobacillus Medium (ATCC Medium 528)