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Thiobacillus perometabolis Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ...................................................................................... 10.0g

Yeast extract.................................................................................. 5.0g

NH4Cl ........................................................................................... 1.0g

K2HPO4......................................................................................... 0.6g

MgCl2............................................................................................ 0.5g

KH2PO4......................................................................................... 0.4g

MgSO4 .......................................................................................... 0.3g

Bromthymol Blue ....................................................................... 0.03g

FeCl3 ........................................................................................... 0.02g

Heavy metal solution ...............................................................30.0mL

pH 3.0–4.0 at 25°C

Heavy Metal Solution:

Composition per liter:

Ethylenediamine tetraacetate ........................................................ 1.5g

FeSO4·7H2O.................................................................................. 0.2g

ZnSO4·H2O ................................................................................... 0.1g

MnCl2·4H2O................................................................................ 0.02g

Modified Hoagland trace elements solution ..............................6.0mL

Preparation of Heavy Metal Solution: Add ethylenediamine tet

raacetate to distilled/deionized water and bring volume to 500.0mL.

Mix thoroughly. Adjust pH to 6.8 to dissolve EDTA. Add remaining

components. Bring volume to 1.0L with distilled/deionized water. Mix

thoroughly. Adjust pH to 6.8.

Modified Hoagland Trace Elements Solution:

Composition per liter:

H3BO3 ......................................................................................... 11.0g

MnCl2·4H2O.................................................................................. 7.0g

AlCl3 ............................................................................................. 1.0g

CoCl2............................................................................................. 1.0g

CuCl2............................................................................................. 1.0g

Kl .................................................................................................. 1.0g

NiCl2 ............................................................................................. 1.0g

ZnCl2............................................................................................. 1.0g

BaCl2............................................................................................. 0.5g

KBr ............................................................................................... 0.5g

LiCl............................................................................................... 0.5g

Na2MoO4 ...................................................................................... 0.5g

SeS2............................................................................................... 0.5g

SnCl2·2H2O................................................................................... 0.5g

NaVO3·H2O .................................................................................. 0.1g

Preparation of Modified Hoagland Trace Elements Solu

tion: Add components seqentially to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3–4.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. A flaky yellow precipitate forms after mixing but will change

in a few days to a fine, white precipitate. Mix the medium thoroughly

before use.

Use: For the cultivation of Thiobacillus perometabolis and other bac

teria that can utilize thiosulfate as an energy source.

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Thiobacillus perometabolis Medium