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Thiobacillus plumbophilus Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NiCl2·6H2O................................................................................... 5.0g

MgSO4·7H2O.............................................................................. 3.45g

MgCl2·6H2O ............................................................................... 2.75g

NH4Cl ......................................................................................... 1.25g

NaCl.............................................................................................. 0.5g

KCl................................................................................................ 0.3g

CaCl2·2H2O ................................................................................ 0.14g

K2HPO4....................................................................................... 0.14g

Na2WO4·2H2O........................................................................... 0.5mg

Trace elements solution ...........................................................10.0mL

pH 6.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O............................................................................... 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl.............................................................................................. 1.0g

MnSO4·2H2O............................................................................... 0.5g

CoSO4·7H2O............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

KAI(SO4)2·12H2O ...................................................................... 0.02g

CuSO4·5H2O............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O ......................................................................... 0.01g

NiCl2·6H2O............................................................................... 0.025g

Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

KOH. Add remaining components. Add distilled/deionized water to

1.0L.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with

H2SO4. Distribute 20.0mL volumes into 100.0mL serum bottles.

Sparge with 80% H2 + 20% CO2. Autoclave for 15 min at 15 psi pres

sure–121°C. Check pH of medium after autoclaving and bring to 6.0 if

necessary. After inoculation add 3% sterile air (9.0mL per serum bot

tle) with a syringe. Pressurize bottles to 2 bar with 80% H2 + 20% CO2.

Use: For the cultivation and maintenance of Thiobacillus plumbophi

lus.

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Thiobacillus plumbophilus Medium