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Thiobacillus prosperus Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

MgSO4·7H2O .............................................................................. 3.45g

MgCl2·6H2O................................................................................ 2.75g

NH4Cl ......................................................................................... 1.25g

NaCl .............................................................................................. 0.5g

Sulfur, powdered........................................................................... 0.5g

KCl.............................................................................................. 0.33g

CaCl2·2H2O................................................................................. 0.14g

K2HPO4....................................................................................... 0.14g

KH2PO4....................................................................................... 0.14g

NiCl2·6H2O ................................................................................2.0mg

Trace elements solution ...........................................................10.0mL

pH 2.5 ± 0.2 at 25°C

Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and

sterilize by steaming for 3 hr on 3 consecutive days.

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ............................................................................... 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·2H2O ............................................................................... 0.5g

CoSO4·7H2O ............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O ............................................................................... 0.025g

KAI(SO4)2·12H2O....................................................................... 0.02g

CuSO4·5H2O ............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O ......................................................................... 0.01g

Na2SeO3·5H2O...........................................................................0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

KOH. Add remaining components. Add distilled/deionized water to

1.0L.

Preparation of Medium: Add components, except sulfur, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 2.5 with H2SO4. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 0.5g of sterile sulfur. Mix thoroughly. Asepti

cally distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus prosperus.

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Thiobacillus prosperus Medium