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Thiobacillus tepidarius Medium

[所属分类:培养基配方] [发布时间:2021-9-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 10.0g

Na2S2O3·5H2O ............................................................................ 4.96g

MgSO4·7H2O ................................................................................ 0.8g

NH4Cl ........................................................................................... 0.4g

Phosphate solution .................................................................100.0mL

Bromcresol Purple, saturated solution .......................................2.0mL

Trace metals A-5........................................................................1.0mL

Phosphate Solution:

Composition per 100.0mL:

KH2PO4......................................................................................... 4.0g

K2HPO4......................................................................................... 4.0g

Preparation of Phosphate Solution: Add components to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Trace Metals A-5:

Composition per liter:

H3BO3 ......................................................................................... 2.86g

MnCl2·4H2O ............................................................................... 1.81g

Na2MoO4·2H2O .......................................................................... 0.39g

ZnSO4·7H2O ............................................................................. 0.222g

CuSO4·5H2O............................................................................. 0.079g

Co(NO3)2·6H2O ....................................................................... 49.4mg

Preparation of Trace Metals A-5: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except phosphate solu

tion, to distilled/deionized water and bring volume to 900.0mL. Auto

clave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL

of the sterile phosphate solution. Mix thoroughly. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus tepidarius.