培养基配方

您现在的位置：网站首页 >> 培养基配方

Thiobacillus thiooxidans Medium

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur, powdered......................................................................... 10.0g

KH2PO4......................................................................................... 5.0g

MgSO4·7H2O................................................................................ 0.5g

CaCl2........................................................................................... 0.25g

(NH4)2SO4 .................................................................................... 0.2g

FeSO4.......................................................................................... 0.01g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except sulfur, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis

tribute into flasks in 100.0mL volumes. Add 1.0g of sulfur to each

flask. Autoclave for 30 min at 0 psi pressure–100°C on 3 consecutive

days.

Use: For the cultivation of Thiobacillus thiooxidans.