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Thiobacillus thiooxidans Medium

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur, powdered......................................................................... 10.0g

KH2PO4......................................................................................... 3.0g

CaCl2·2H2O................................................................................. 0.14g

MgCl2·6H2O.................................................................................. 0.1g

NH4Cl ........................................................................................... 0.1g

pH 4.2 ± 0.2 at 25°C

Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and

sterilize by autoclaving for 15 min at 8 psi pressure–112°C.

Preparation of Medium: Add components, except sulfur, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 2.5 with H2SO4. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically layer 0.5g of sterile sulfur onto the surface of the

medium.

Use: For the cultivation and maintenance of Thiobacillus species.